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vol.3 número2Semi-quantitative detection of genetically modified grains based on CaMV 35S promoter amplificationMitotic aberrations in coffee (Coffea arabica cv. 'Catimor') leaf explants and their derived embryogenic calli índice de autoresíndice de materiabúsqueda de artículos
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Electronic Journal of Biotechnology

versión On-line ISSN 0717-3458


CUCCO, María Florencia  y  ROSSI JAUME, Alberto Daniel. Protocol for regeneration in vitro of Arachis hypogaea L. Electron. J. Biotechnol. [online]. 2000, vol.3, n.2, pp.35-50. ISSN 0717-3458.

The regeneration of peanut Runner varieties grown in the South of the province of Cordoba, Argentina, was studied to count on a regeneration protocol, which is essential to perform genetic transformation of the plant. The induction of somatic embryos was evaluated in different peanut explants cultivated in Cordoba: cotyledons with and without embryos, epycotils and leaflets, of the Tegua, Nahuel and Florman INTA varieties. The induction medium consisted of MS salts with 30 g.l-1 sucrose, supplemented with the following hormones and herbicides: BAP, Picloram, DMA and 2,4-D. The highest percentage of embryogenic callus was produced cultivating epicotyls with 50 mg.l-1 2,4-D. The embryos obtained produced plantlets which, once acclimatized, reached the reproductive stage.

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