SciELO - Scientific Electronic Library Online

vol.4 número3Molecular dynamics simulations of active site mutants of rat liver arginasePlant genomics and agriculture: From model organisms to crops, the role of data mining for gene discovery índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados


Electronic Journal of Biotechnology

versión On-line ISSN 0717-3458


ZALDIVAR, Mercedes; VELASQUEZ, Juan Carlos; CONTRERAS, Inés  y  PEREZ, Luz María. Trichoderma aureoviride 7-121, a mutant with enhanced production of lytic enzymes:: its potential use in waste cellulose degradation and/or biocontrol. Electron. J. Biotechnol. [online]. 2001, vol.4, n.3, pp.13-14. ISSN 0717-3458.

A mutant of the native fungus Trichoderma aureoviride, 7-121, selected for its overproduction of extracellular cellulase and ß-glucosidase (cellobiase) was obtained. In shake flask cultures, production of endoglucanase, filter paper activity and cellobiase increased two to four- fold as compared with the wild type strain. The mutant strain is stable and grows rapidly in liquid as well as in solid culture media. Enzyme yields were best when pH was controlled so that it did not fall bellow pH 3.5. Cellobiase production by this mutant is particularly high (approximately 5 U/ml) as compared to other Trichoderma, strains, which makes it a suitable candidate for waste cellulose degradation. In addition, the mutant strain showed enhanced production of fungal cell wall degrading enzymes: chitinases, ß-1,3-glucanases and proteases. This improvement in extracellular enzyme production by the mutant T. aureoviride 7-121 suggests that it is a suitable strain to be used in biological control.

Palabras clave : .

        · texto en Inglés


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons