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Electronic Journal of Biotechnology

versión On-line ISSN 0717-3458


SIVAPRAKASAM, Senthilkumar; MAHADEVAN, Surianarayanan  y  GOPALARAMAN, Swaminathan. Oxygen mass transfer studies on batch cultivation of P. aeruginosa in a biocalorimeter. Electron. J. Biotechnol. [online]. 2008, vol.11, n.1, pp.80-92. ISSN 0717-3458.

In the present work volumetric mass transfer coefficient (kLa) was investigated during batch cultivations of Pseudomonas aeruginosa on a nutrient media. The effects of process variables (viz. impeller speed, oxygen flow and geometry of impeller) on the volumetric mass transfer coefficient of oxygen, kLa, in a biocalorimeter (Bio-RC1) was investigated and reported in this research work. The experimental data have been analyzed employing MATLAB to obtain the influences of the process parameters on kLa. An attempt was made to correlate volumetric mass transfer coefficient with metabolic heat production rate at optimized process conditions. The correlation reported in this work would be useful to control and scale up of bioprocesses.

Palabras clave : biocalorimeter; metabolic heat; P. aeruginosa; scale-up; volumetric mass transfer coefficient.

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