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Electronic Journal of Biotechnology

versión On-line ISSN 0717-3458


GODOY-HERNANDEZ, Gregorio; AVILES-BERZUNZA, Elidé; CARRILLO-PECH, Mildred  y  VAZQUEZ-FLOTA, Felipe. Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.). Electron. J. Biotechnol. [online]. 2008, vol.11, n.1, pp.134-139. ISSN 0717-3458.

Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation

Palabras clave : Argemone mexicana; β-glucuronidase; benzylisoquinoline alkaloids; genetic transformation; neomycin phosphotransferase II.

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