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vol.14 número1A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilagesSelection of reference genes for normalization of quantitative real-time PCR in cell cultures of Cyclamen persicum índice de autoresíndice de assuntospesquisa de artigos
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Electronic Journal of Biotechnology

versão On-line ISSN 0717-3458


PAVLOV, Maria Soledad et al. Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects. Electron. J. Biotechnol. [online]. 2011, vol.14, n.1, pp.11-12. ISSN 0717-3458.

A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody.

Palavras-chave : digital image analysis; fluorescence microscope methods; immunofluorescence; Infectious pancreatic necrosis virus (IPNV); SYBR-Green I.

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