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Electronic Journal of Biotechnology

versión On-line ISSN 0717-3458

Resumen

WANG, Chuan Tang et al. Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content. Electron. J. Biotechnol. [online]. 2011, vol.14, n.2, pp.10-10. ISSN 0717-3458.

Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity.

Palabras clave : chemical mutant; FAD2B; NIRS; oleate; peanut.

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