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Electronic Journal of Biotechnology

versión On-line ISSN 0717-3458

Resumen

KIM, Jong-Wan; YOE, Jeehyun; LEE, Gil Ho  y  YOE, Sung Moon. Recombinant expression and refolding of the c-type lysozyme from Spodoptera litura in E. coli. Electron. J. Biotechnol. [online]. 2011, vol.14, n.3, pp.6-6. ISSN 0717-3458.

The chicken-type lysozyme of the insect Spodoptera litura (SLLyz) is a polypeptide of 121 amino acids containing four disulfide bridges and 17 rare codons and participates in innate defense as an anti-bacterial enzyme. The recombinant S. litura lysozyme (rSLLyz) expressed as a C-terminal fusion protein with glutathione S-transferase (GST) in Rosetta(DE3) Singles. The protein was produced as an inclusion body which was solubilized in 8 M urea, renatured by on-column refolding, and purified by reversed-phase chromatography to 95% purity. The purified rSLLyz demonstrated antibacterial activity against B. megaterium confirmed by inhibition zone assay. The overexpression and refolding strategy described in this study will provide a reliable technique for maximizing production and purification of proteins expressed as inclusion bodies in E. coli.

Palabras clave : antibacterial activity; inclusion body; lysozyme; on-column refolding; recombinant expression; Spodoptera litura.

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