SciELO - Scientific Electronic Library Online

vol.15 número2Intein-mediated expression of cecropin in Escherichia coliImproved isolation of good-quality total RNA from the optic stalk of Mud crab, Scylla paramamosain índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados


Electronic Journal of Biotechnology

versión On-line ISSN 0717-3458


MOE, Kyaw Thu; HONG, Woo-Ju; KWON, Soon-Wook  y  PARK, Yong-Jin. Development of cDNA-derived SSR markers and their efficiency in diversity assessment of Cymbidium accessions. Electron. J. Biotechnol. [online]. 2012, vol.15, n.2, pp.4-4. ISSN 0717-3458.

Cymbidium spp. are popular flowering plants. Assessment of the genetic diversity in cultivated Cymbidium facilitates conservation of germplasm and subsequent cultivar improvement. Thus, it is important to develop more efficient polymorphic DNA markers. Although more motifs (403) were identified and more primers (206) were designed in the genomic library compared to the cDNA library, a larger number of successful primers were obtained from the cDNA library (59.9%) than from genomic DNA library (51.1%). However, higher PIC and gene diversity were identified in genomic SSRs. The average allele number per locus was also higher in genomic SSRs (7.3) than EST-SSRs (5.2), among the 24 evaluated Cymbidium accessions. AT/TA was comparatively high in EST-SSRs, while this motif was not as common in genomic SSRs. The CTT/AAG/TCT/AGA/TTC/GAA and TGC/GCA/GCT/AGC/CTG/CAG motifs were the most abundant tri-nucleotide sequences in EST-SSRs, while GTT/AAC/TGT/ACA/TTG/CAA was the most frequent in genomic SSRs. The number of repeats ranged from 3 to 12 in EST-SSRs. Currently, 52 novel polymorphic SSR markers have been evaluated, which will be useful for germplasm assessments, core set construction, evaluation of genetic diversity, and marker assisted selection (MAS) based Cymbidium breeding.

Palabras clave : cDNA library; Cymbidium; enriched library; molecular diversity; SSR.

        · texto en Inglés     · Inglés ( pdf )


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons