SciELO - Scientific Electronic Library Online

vol.15 número3Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strainsXylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados




Links relacionados


Electronic Journal of Biotechnology

versão On-line ISSN 0717-3458


AHMED, Sibtain; IMDAD, Syeda Sana  e  JAMIL, Amer. Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum. Electron. J. Biotechnol. [online]. 2012, vol.15, n.3, pp.3-3. ISSN 0717-3458.

Xylanases assume special importance in the paper and pulp industry as they replace toxic chemicals such as chlorine and chlorine dioxide for developing eco-friendly processes. This study evaluated xylanases produced by two fungi, the mesophilic fungus Trichoderma harzianum and a thermophilic fungus Chaetomium thermophilum. Among the polymeric substrates studied for xylanase production by both the fungi, birch wood xylan was found to be the best inducer of xylanases. Xylanases induction was subject to glucose repression. Partially purified xylanases preparation from T. harzianum and C. thermophilum exhibited optimal activities at pH 5 and 6 and at 60ºC and 70ºC, respectively. The apparent Km and Vmax values for the partially purified xylanase from T. harzianum using oat spelt xylan as a substrate were 4.8 mg mL-1 and 0.526 µmol min-1 mg-1, respectively. Whereas values of the partially purified xylanase from C. thermophilum were 2.96 mg mL-1 and 0.25 µmol min-1 mg-1, respectively. These findings in this study have great implications for the future applications of xylanases.

Palavras-chave : C. thermophilum; kinetics; T. harzianum; xylanase.

        · texto em Inglês     · Inglês ( pdf )


Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons