SciELO - Scientific Electronic Library Online

vol.18 issue3Study on the relationship between intracellular metabolites and astaxanthin accumulation during Phaffia rhodozyma fermentationStable expression and characterization of a fungal pectinase and bacterial peroxidase genes in tobacco chloroplast author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google


Electronic Journal of Biotechnology

On-line version ISSN 0717-3458


LOPES COSTA, Cecília Ladeira  and  COLLI BADINO, Alberto. Overproduction of clavulanic acid by extractive fermentation. Electron. J. Biotechnol. [online]. 2015, vol.18, n.3, pp.154-160. ISSN 0717-3458.

Background Clavulanic acid is an important beta-lactamase inhibitor produced as a secondary metabolite by the actinomycete Streptomyces clavuligerus. Clavulanic acid is chemically unstable; therefore, it is degraded during bacterial cultivation. In this work, the adsorbents clinoptilolite, activated carbon, calcined hydrotalcite, and Amberlite IRA 400 anionic exchange resin were studied in terms of their ability to adsorb clavulanic acid during extractive fermentation, in order to prevent product degradation and avoid product concentrations reaching inhibitory levels. Adsorption assays were used to investigate the effect of pH, and the decrease in the clavulanic acid concentration in the culture broth was measured for each adsorbent. Results IRA 400 was found to be most effective, with 78% adsorption of clavulanic acid. The maximum production of clavulanic acid in Erlenmeyer flask cultures increased 86% in terms of mass of CA, and 248% in cumulative CA concentration, with the use of Amberlite IRA 400 as adsorbent in extractive fermentation, compared to control fermentation performed without product removal. Conclusions The results indicated that extractive fermentation using a solid phase could be an important way of enhancing clavulanic acid titers. It was also possible to show that clavulanic acid acts as an inhibitor of its own synthesis.

Keywords : Adsorption; Clavulanic acid; Extractive fermentation; Integrated processing; Product inhibition.

        · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License