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Gayana (Concepción)

Print version ISSN 0717-652XOn-line version ISSN 0717-6538

Abstract

ALAYO, Marianella  and  IANNACONE, José. ECOTOXICOLOGICAL ASSAY WITH CRUDE OIL, DIESEL 2 AND DIESEL 6 WITH TWO SUBSPECIES OF BRACHIONUS PLICATILIS MÜLLER, 1786 (ROTIFERA: MONOGONONTA). Gayana (Concepc.) [online]. 2002, vol.66, n.1, pp.45-58. ISSN 0717-652X.  http://dx.doi.org/10.4067/S0717-65382002000100007.

ABSTRACT We conducted standardized ecotoxicological assay with the rotifer Brachionus plicatilis rotundiformis line S and B. plicatilis hepatotomus line L took as tools to evaluate aquatic environment, employing the Water-Soluble Fraction (WSF) of three hydrocarbons: Crude oil (CO), Diesel 2 (D2) and Diesel 6 (D6). The values determined of LC50 for B. plicatilis rotundiformis at 24 and 48 h exposure of WSF from CO, D2 and D6 were 0,13, 0,04; 0,65, 0,14 and 4,20, 0,33 mg/L, respectively. The values determined of CL50 for B. plicatilis hepatotomus at 24 and 48 h exposure of WSF from CO, D2 and D6 were 0,23, 0,05; 1,36, 0,13 and 3,47, 1,01 mg/L, respectively. Among the three hydrocarbons evaluated the order of ecotoxicity decreased at 24 and 48 h exposure for both subspecies were: Crude oils > Diesel 2 > Diesel 6. We did not find differences between the sensibility of both subspecies of Brachionus. We proposed a protocol for the bioassay employing B. plicatilis rotundiformis and B. plicatilis hepatotomus as a tool to evaluate aquatic environment. This specie shows the next advantages: wide distribution in marine environment, trustworthily, repeatedly, short last of the bioassay and high sensibility when we compared microcrustaceans as nauplii of Artemia salina and zoeas of Emerita analoga

Keywords : Ecotoxicity; rotifer; Brachionus; petrol; bioassays; water- soluble fraction.

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