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vol.71 número2AMBITO DE HOGAR DE LIOLAEMUS MELANOPS BURMEISTER, 1888 (Squamata: Liolaemini) EN EL CENTRO DE CHUBUT, ARGENTINA índice de autoresíndice de materiabúsqueda de artículos
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Gayana (Concepción)

versión impresa ISSN 0717-652Xversión On-line ISSN 0717-6538

Resumen

SILVA, Jeannette; FUENTEALBA, Carmen; BAY-SCHMITH, Enrique  y  LARRAIN, Alberto. STANDARIZATION OF THE ACUTE TOXICITY BIOASSAY WITH DIPLODON CHILENSIS USING A REFERENCE TOXICANT. Gayana (Concepc.) [online]. 2007, vol.71, n.2, pp.135-141. ISSN 0717-652X.  http://dx.doi.org/10.4067/S0717-65382007000200001.

A wide range of species vulnerability to toxicans makes necessary to use a combination of different species in environmental toxicity assessment. Species specific bioassays are standarized to minimize variations in successive measurements. The freshwater bivalve molluscs Diplodon chilensis is a potential test species for toxicity bioassay based on its widespread and abundant populations in rivers and lakes of Chile and its ecological significance in the plancktonic community as a powerful filter feeder. A standardized procedure with D. chilensis is herein described followed by the method's calibration. Juveniles of this species were exposed to a concentration gradient of potassium dichromate (K2Cr2O7) as reference toxicant for 96 h. The measured effect was mortality. After eight successive estimations of the median lethal concentration (LC50-96 h) a mean value ± SD of 20,4 ± 3,3 mg L-1 was found. Intra laboratory precision, estimated by means of the variation coefficient (VC), was 16,2 % and the superior and inferior control limits for future LC50 values derived from the control chart were 27 and 17,1 mg L-1 respectively. This bivalve can then be used as test organism in ecotoxicity assessment increasing the diversity of species in environmental testing batteries, with the extra benefit of being a mollusc, zoological group poorly represented among freshwater testing animals in ecotoxicological bioassays.

Palabras clave : Freshwater bivalves; calibration; variation coefficient; potassium dichromate.

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