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vol.71 número2AMBITO DE HOGAR DE LIOLAEMUS MELANOPS BURMEISTER, 1888 (Squamata: Liolaemini) EN EL CENTRO DE CHUBUT, ARGENTINAESTRUCTURA DE LA MACROFAUNA EN AMBIENTES OXICOS DE BAHIA COLIUMO, REGION DEL BIO-BIO, CHILE CENTRAL índice de autoresíndice de materiabúsqueda de artículos
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Gayana (Concepción)

versión impresa ISSN 0717-652Xversión On-line ISSN 0717-6538

Resumen

CAMPOS, Víctor et al. ISOLATION OF ARSENIC RESISTANCE BACTERIA FROM VOLCANIC ROCKS OF QUEBRADA CAMARONES, PARINACOTA REGION: CHILE. Gayana (Concepc.) [online]. 2007, vol.71, n.2, pp.150-155. ISSN 0717-652X.  http://dx.doi.org/10.4067/S0717-65382007000200003.

Arsenic is naturally present in rocks, soil, water, and air, being released to the environment by natural processes such as volcanic eruptions, and erosion rock. Microorganisms are known to play an important role in the Arsenic natural cycle. The aim of this work was isolate arsenic resistant bacteria to volcanic rocks, from Quebrada Camarones, Parinacota Region, Chile. Rocks were cultured in an arsenite conditioned mineral broth (500 ug/mL) over 7 days at ambient temperature, under stirring. Strains were isolated using different medium and identified by Rapid™NF plus system. The arsenic oxidation capacity was assayed with silver nitrate, and aox genes were detected by RT-PCR. Arsenic reduction was evaluated means ars gene amplification by PCR. Gram negative no fermentative bacillum, identified as Pseudomonas alcaligenes and Wautersia solanacearum able to tolerate concentrations above 8 mM As(III) were isolated. RT-PCR analysis showed the presence of aox, genes; these codify an oxidant enzyme that catalyses As(III) oxidation to As(V). The Arsenic oxidation capacity of the isolated seeds would favor colonization by other non tolerant species participating in biogeochemical cycles

Palabras clave : Arsenite; arseniate; Wautersia solanacearum; Pseudomonas alcaligenes.

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