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Investigaciones marinas

On-line version ISSN 0717-7178

Abstract

GOMEZ, Daniel  and  DUPRE, Enrique. Terminal monosaccharid on the oocyte envelope of the shrimp rock Rhynchocinetes typus (Crustacea, Decapoda). Investig. mar. [online]. 2002, vol.30, n.2, pp.69-74. ISSN 0717-7178.  http://dx.doi.org/10.4067/S0717-71782002000200007.

Oligosaccharids of glycoproteins present in oocytes envelope during the first gamete interaction have an important role in the sperm recognizing process. The terminal monosaccharid of oligosaccharids can be recognized by specific lectins. The number and location of monosaccharid on the oocyte coat changes in many marine species according to the oocyte maturation. This study determines the presence of monosaccharids on the oocyte envelope and its location during the oocyte maturation by the following specific FITC-labeled lectins: LCA, BS-1, LTA and Con-A which recognize the mannose, galactose, fucose and glucose monosaccharid respectively. The results of the lectins experiments showed a high concentration of mannose (2-3 time) on the mature oocyte coat. From 5 days pre-molt to 2 day post-molt he concentration of mannose, galactose and glucose increasing on the oocyte envelope increaded. The fucose monosacharide was 3 time less than mannose. This results suggest that mannose could be participating in the first gametic interaction., however the involvement of these carbohydrates during the fertilization must be determined by in vitro fertilization inhibition experiments

Keywords : carbohydrates; vitelline coat; chorion; oocyte; shrimp.

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