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Investigaciones marinas

On-line version ISSN 0717-7178


JOO, Roberto  and  DUPRE, Enrique. Effect of different cryoprotectants on the sperm motility of the surf-clam Mesodesma donacium (Mollusca, Bivalvia). Investig. mar. [online]. 2002, vol.30, n.2, pp.75-79. ISSN 0717-7178.

Toxicity of DMSO, methanol and prophylene glycol at 0.5 M, 1.0 M and 1.5 M at 5, 10 and 15 min of incubation time are analyzed. The spermatozoa removed from the gonad where diluted in 5ºC seawater and later mixed in proportion 1:1 with the corresponding cryoprotectants. The toxicity generated by the cryoprotectants, after the incubation were evaluated by sperm motility. Three type of motility were differentiated (M1, M2 y M3). The fresh spermatozoa were activated at contact with seawater increasing flagellar movement until 15 min and them diminished until to 95 min. After the incubation of the sperm in each one cryoprotectant, the highest recovery of forward motility (type 3) was 44.3% at DMSO 1.0 M incubated during 5 min, which suggest that DMSO produce the low toxicity. Though the molecules of the other cryoprotectant are smaller and could easier penetrate into de cell, they can cause a faster dehydration and thereby destruction of the membranes by shrinking or could be affecting directly the flagellar structures

Keywords : toxicity; cryoprotectant; cryopreservation; spermatozoa; surf- clam.

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