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Investigaciones marinas

On-line version ISSN 0717-7178


DUPRE, Enrique  and  JOO, Roberto. Viability of cryopreserved spermatozoa of the surf clam Mesodesma donacium (Mollusca, Bivalvia). Investig. mar. [online]. 2006, vol.34, n.2, pp.187-190. ISSN 0717-7178.

The viability of the surf clam spermatozoa was evaluated using the percentage of fertilization obtained with cryopreserved spermatozoa and different protocols. In total, 42 protocols combined three types of cryoprotectants (DMSO, Methanol, and Prophylen-glycol) at three different concentrations (0.5 M, 1.0 M, and 1.5 M), four freezing rates (-5, -10, -15, and -206ºC·min-1), and two thawing rates (slow: 72ºC·min-1 and fast: 312ºC·min-1). The best sperm motility was obtained with DMSO frozen at -15ºC·min-1 and with a slow thawing rate. The best fertilization percentages were obtained with DMSO frozen at -15ºC·min-1 but with a fast thawing rate

Keywords : cryopreservation; spermatozoa; fertilization; mollusk; bivalve.

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