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International Journal of Morphology

On-line version ISSN 0717-9502


PARRA LARA, Roxana; MATAMALA VARGAS, Fernando  and  SILVA MELLA, Héctor. Morphological Effect of AsGa Laser Irradiation on Rat Skeletal Muscle . Int. J. Morphol. [online]. 2007, vol.25, n.1, pp.43-50. ISSN 0717-9502.

Whether laser therapy is widely used in healing therapies of injured muscle, there are no reports about its morphological effects on healthy muscle. A morphometrical and stereological study was performed with healthy Sprague Dawley rats, all irradiated with AsGa laser directly on the skin of the gastrocnemius muscle belly area for ten days, using three different daily doses on groups of five rats 0,8 (Al y A2), 2 (B) y 5 (C) joules/cm2 on the left posterior limb. The right limb was no irradiated, so it was used as control. The muscle fiber area was determinate by point counting (morphometry) on group At. Density of connective tissue contain (Vvtc) was determinate by M42 test (stereology) on A2, B and C group. The analysis of all the 500 fiber areas obtained from At group muscles and 500 fiber areas from its control muscles show that the mean fiber area was 1513±376 mm2 for laser treated gastrocnemius muscles and 1146±286 mm2 for non irradiated control gastrocnemius muscles. Regression analysis showed that the fiber area was higher on treated muscles (p<0,001). Stereological analysis of Vvtc results showed no significance difference between treated and control muscles in none of the used doses (p=0.07). AsGa laser therapy produce morphological effects on healthy muscle

Keywords : Skeletal muscle; Laser therapy; Gastrocnemius muscle.

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