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International Journal of Morphology

versión On-line ISSN 0717-9502

Resumen

ROSAS C, Carlos; VASQUEZ, Bélgica  y  DEL SOL, Mariano. Stereological Study of the Guinea-pig Liver (Cavia porcellus). Int. J. Morphol. [online]. 2007, vol.25, n.3, pp.557-560. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022007000300014.

The liver is a widely researched organ for having an infinity of functions. On the other hand, Stereologyallows the access of quantitaive data of the study´s structure. Having access to this tpe of date of hepatocitos and sinusoids Under normal physiological conditions, it is necessary to determine how the number of these cells varies in pathological conditions. The principal objective of this investigation was to describe the morphology of the liver of cobayo, through Stereology, setting the bases for future morphofuncional investigations. Five adult males of the Cavia porcellus species were used, of which the liver was extracted and its volume determined, applying the Scherle method. Subsequently five pieces of aleatoria form were obtained which were set in an aqueous Bouin solution. Of these samples 3µm cuts were obtained, that were dyed with hematoxilina-eosina. For the stereological study the M42 system was used. The following were determined: the number densities, volume and surface and total number of hepatocitos and sinusoides. An index of lesser significance or equal to 0,05. The NV of hepatocitos was9.20 x 105 /mm3, VV de 70.04% and SV of 254.95 mm2/mm3. In the case of the sinusoides NV was 1.94 x 105 /mm3, VV of 24.86% and SV de 47.01 mm2/mm3. The total number of hepatocites was of 2.8 x 107 and sinusoides 0.61 x 107. While comparing the results obtained in Cavia porcellus and those obtained in other species, a quantitative variation in the hepatic morphology is observed. With these results it is possible to establish the bases for experimental research

Palabras clave : Liver; Guinea pig; Cavia porcellus; Stereology.

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