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International Journal of Morphology

versión On-line ISSN 0717-9502

Resumen

RAI, Anju Lata  y  JESWAR, Usha. Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus. Int. J. Morphol. [online]. 2010, vol.28, n.2, pp.483-487. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022010000200024.

The present study was planned to demonstrate the detailed immunoreactive (IR) distribution pattern of estrogen receptors (ER) in hippocampus of 15 female rats, adult, female Wistar rats in estrous phase. 30 µm thick setions of hippocampal region fixed (4% buffered paraformaldehyde) were obtained with cryostat. The sections were processed free- floating for immunolocalization of ER using, mouse monoclonal anti-ER-a antibody with PAP technique. The results showed presence of ER immunoreactive neurons in all the subfields of hippocampus with some variations. In cornua ammonis (CA) maximum ER positive (+ve) neurons were localized in CA3 region. Layer analysis showed maximum localization in the stratum oriens (SO) region. In other subfields and layers of CA the IR neurons were comparatively less in number. The morphological characters of all ER +ve neurons showed them to be interneurons both in CA as well as in Dentate gyrus (DG).

Palabras clave : Estrogen receptor-a; Estrogen receptor-b; Brain; Hippocampus; Interneurons; Cornua ammonis.

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