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International Journal of Morphology

versión On-line ISSN 0717-9502

Resumen

VALDES, Fabio; VEGA, Eduardo  y  VALENZUELA, Marcos. Two Different Plastination Techniques Comparative Study. Int. J. Morphol. [online]. 2010, vol.28, n.3, pp.783-786. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022010000300020.

The plastination technique is used extensively for the improved preservation of cadavers used for teaching. Our unit has been using this technique since 2002, but we have noticed that sample quality is not optimal, given the large retraction of samples. Therefore, a project was designed that compares our department's plastination technique, with another process, based on the protocol used by Murcia University. We used 24 fresh bovine kidneys and brain samples, pigs' kidneys and hearts and human lower limbs; all of which were fixed with ethyl alcohol, formalin and De los Andes University's fixation solution. Two different plastination techniques were applied: one based on the protocol used in our laboratory (technique A) and another one similar to that published by Murcia University (technique B). We evaluated the body weight percentage, width, length, thickness and perimeter of the different plastinated pieces, as well as measuring the same variables in each fixation solution (alcohol, formalin fixative solution 10% and Universidad de los Andes). The average weight loss percentage was 63.1% for technique A and 37.9% for technique B (p <0.01). The decrease measurements in length, width and thickness were also higher under technique A, all percentages being statistically significant. When comparing the samples according to different fixation methods, there were no significant differences. Plastination based on Murcia University's technique obtained better quality samples with less shrinkage. The plastination process was not influenced by the fixation method.

Palabras clave : Tissue Preservation; Silicone; Acetone; Vaccum.

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