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International Journal of Morphology

On-line version ISSN 0717-9502

Abstract

SPOSITO, Danilo Rodrigues  and  SANTOS JR, Arnaldo Rodrigues. Histochemical Study of Early Embryo Implantation in Rats. Int. J. Morphol. [online]. 2011, vol.29, n.1, pp.187-192. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022011000100032.

Embryo implantation is the process that results in attachment of the conceptus to the uterine wall. In this histochemical study, we investigated the early stage of embryo implantation in rats by morphological analysis and by the detection of total proteins and glycosaminoglycans using hematoxylin-eosin, toluidine blue at pH 4.0 (TB), and Xylidine Ponceau at pH 2.5 (XP). In non-pregnant females, the uterine layers could be clearly distinguished and showed the normal histology of the organ. In pregnant females, an increase in the number of cells and a reduction in the interstitial space were observed in the endometrium close to the implantation sites. The blastocyst was partially inserted in the endometrium, with the observation of the inner cells mass around the blastocyst cavity surrounded by trophoblastic cells. TB staining revealed mild metachromatic basophilia, which was more evident in the endometrial stroma around the implantation site. Histochemical staining with XP was also more intense in the stroma close to the site of implantation. On the other hand, histochemical staining with either TB or XP was more discrete at sites distant from the conceptus. This study demonstrated changes in the endometrial stroma in areas adjacent to the site of embryo implantation, with variations in glycosaminoglycans and proteins as demonstrated by the detection of anionic and cationic radicals, respectively.

Keywords : Implantation window; Endometrium; Trophoblast; Embryo.

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