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International Journal of Morphology

On-line version ISSN 0717-9502


ESPINOZA-NAVARRO, O  and  SARABIA, L. Evaluation and Standardization of the Quality of Semen: New Lower Reference Limit. Int. J. Morphol. [online]. 2011, vol.29, n.3, pp.885-890. ISSN 0717-9502.

Spermogram or semen analysis is one of the most used parameters in the evaluation of male fertility.WHO (2010) presented the 5th Manual for review and processing of human semen, a document that was discussed during the first workshop of standardization of semen analysis (PLEAS), held in Santiago de Chile, May 2010. Subsequently appliedthenew values expressed in "lower referencelimit" (LRL) for semen in several analysisstudiesconductedbyvariousauthors (2003 to 2010). The results indicate that 81% of Latin American researchers believe the new manual standardizes best sperm concentration, 96% agree with the new subclassification in progressive sperm motility (A), non-progressive (B) andimmobile (C). 68% determined that the best instruments for the sperm countis the Neubauer haemocytometerchamber. Regarding quality control only 18% performed external quality control. 100% ofresearchersbelieveit isappropriate to conductongoing standardization workshops. Regarding the application of LRL in the study populations (2003-2010), 100% comply with the standards to be considered a population with fertility capacity. However, several authors argue that a new version of the WHO manual, must be re-done urgently to better standardize sperm concentration (15 million/mL) and morphology according to strict criteria (4%), reference values considered very low.

Keywords : Semen analysis; Spermiogram; Male fertility; Lower reference limit.

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