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International Journal of Morphology

On-line version ISSN 0717-9502

Abstract

OCHOA-AMAYA, J. E et al. Systemic Toxoplamosis in a Captive Red Kangaroo (Macropus rufus). Int. J. Morphol. [online]. 2012, vol.30, n.1, pp.70-76. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022012000100012.

The objetives of this study were to present and document the hystopathologycal findings of systemic toxoplamosis in a captive red kangaroo (Macropus rufus) which described macro and microscopic findings of the hystopathological analysis. In the laboratory of animal histopathology (Universidad de los Llanos) formalin fixed tissue specimens were received, from a captive male Macropus rufus, who was eight years old and weighed 50 kg. The samples were processed by usual methods for optical microscopy. The histological sections of 3-4 mm thick were colored with Hematoxilin-Eosin (H&E) and then some samples stained with Periodic Acid Schiff (PAS), and processed by PCR and IHQ. Once the histopathological analysis was performed systemic toxoplasmosis was associated to protozoa cysts immunoreactives to T. gondii. The molecular detection of T. gondii in formalin fixed tissues was made using two PCR tests and confirmated by IHQ. Histopathologically a chronic infection by an eucoccideo protozoa from the Sarcocystidae family was diagnosed. The etiologic diagnosis was toxoplasmosis.

Keywords : Kangaroo; Toxoplasmosis; Tachyzoites; Bradyzoites.

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