SciELO - Scientific Electronic Library Online

 
vol.30 issue4Stem Cells in Development and the Perspectives of Cellular Reprogramming for RegenerationMorphological Diversity of the Ovaries of the Mexican Teleost Fishes author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


International Journal of Morphology

On-line version ISSN 0717-9502

Abstract

HIPKAEO, Wiphawi et al. Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid?. Int. J. Morphol. [online]. 2012, vol.30, n.4, pp.1348-1352. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022012000400016.

After careful observation of immuno-electron microscopic findings of the renal glomerular podocyte slit so far published by various authors, it should be noted that no convincing evidence is present for localization of nephrin-immunoreactivity in the slit itself, but that nephrin-immunoreactivity is dominantly localized along the membranes of podocyte pedicles from their tip over a substantial extension. In addition, the notion that the slit diaphragm is composed of strands is compatible with the widely distributed view that the slit strands represent rod-shaped nephrin molecules. However, 3-D findings that the diaphragm is composed of not only strands but also membranous elements have recently demonstrated separately by embedment-free section electron microscopy as well as by frozen/deep-etched replica electron microscopy. It is therefore suggested that the molecular composition of the slit diaphragm remain to be further elucidated, and that it is also necessary to re-consider how nephrin is involved in the renal glomerular filtration based on the exact localization along the podocyte membrane, but not in the slit itself. The present author would welcome any criticism and opinion on his consideration.

Keywords : Glomerulus; Slit; Nephrin; Localization; Immunohistochemistry.

        · abstract in Spanish     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License