SciELO - Scientific Electronic Library Online

 
vol.31 issue1Relationship between Zoometric Measurements in Holstein-Friesian Cow and Cubicle Size in Dairy FarmsContributions of Wilhelm His to Human Embryology author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


International Journal of Morphology

On-line version ISSN 0717-9502

Abstract

DUMAN, Selcuk et al. Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue. Int. J. Morphol. [online]. 2013, vol.31, n.1, pp.64-69. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022013000100009.

The aim of adipose tissue engineering is creating autologus vascularized fat tissue to be used for practical soft tissue reconstruction in human clinic. Unfortunately, in practice, long-term results of fat transplantation are often untrustworthy and unreliable, to overcome this problem different many lipoinjection techniques developed in the last 20 years. Centrifuge is a fundamental stepin the preparation of adipose tissue. We focused on some cell markers especially MSCs markers and histological structural properties after with lipokit centrifugation and without lipokit centrifugation of adipose tissue obtained by liposuction by this new technique. Adipose tissue was taken by liposuction and separates to two portions. One of them is centrifugated by Lipokit machine (C+) has a micro filter and the other is not (C-). After centrifugation smear slides and paraffin sections were prepared from these tissues. These slides were stained with H&E and Toluidine Blue. Paraffin sections were immunohistochemically stained with CD34, von Willebrand Factor, CD73, CD90 and CD105. Smear preparations showed a continuous three dimensional plasma membrane appearance of adipocytes. C+ and C- showed expression of CD34, von Willebrand Factor, CD73, CD90, CD105. C+ seems to have more free cells expressing than C-. While passing the filter of Lipokit, large adipocytes and connective tissue parts disintegrate and thus increases the surface area of lipoaspirate. Lipokit® machine release the group cells which are necessary for angiogenesis and they become more freely to construct angiogenesis.

Keywords : Adipose tissue; Stem cell; Angiogenesis.

        · abstract in Spanish     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License