SciELO - Scientific Electronic Library Online

 
vol.31 issue1Determination of Lateral Ventricle and Brain Volume in Children with Stereological Method Using MRISomatotype Profile of Professional Male Soccer Chilean Players author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


International Journal of Morphology

On-line version ISSN 0717-9502

Abstract

SALINAS, Paulo; SANCHEZ, Raúl  and  RISOPATRON, Jennie. Cryopreservation of Canine Spermatozoa at -80C. Int. J. Morphol. [online]. 2013, vol.31, n.1, pp.217-224. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022013000100036.

The freezing and storage in liquid nitrogen (LN2) is the technique used most for the preservation of canine and other domestic species semen, for long periods of time. This study was designed to validate the use of deep freezer at -80C to freeze and store canine semen in straws. Three protocols were tested (n = 10): Control: sperm frozen in N2L and stored; Exp 1: sperm frozen and stored -80°C, and Exp.2: semen frozen at -80 ° C and stored in N2L. Post-thawing was assessed by flow cytometry and the viability of plasma membrane integrity (SYBR-14/PI), mitochondrial membrane potential (YDm, JC-1), acrosome membrane integrity (PSA / FITC-PI), translocation of phosphatidylserine (Annexin -V-FITC/PI) and DNA fragmentation (TUNEL). No significant differences (P <0.05) between the Control, Exp.2 and Exp.1groups regarding: intact plasma membrane integrity (42.2 ± 5.3, 35.57 ± 10.3 and 40.76 ± 12.1, respectively), YDm normal (54.7 ± 20.6, 44.4 ± 15.8 and 43.4 ± 15.3, respectively), intact acrosomal membrane integrity (42.9 ± 11 , 1, 53.2 ± 15.8 and 48.7 ± 20.1, respectively) and fragmented DNA (0.87 ± 0.41, 0.75 ± 0.16, 0.79 ± 0.27, respectively). However, the average motility of the post-thawing of Exp.1 and Exp.2 groups (37.0 ± 4.4% 36.8 ± 4.1% respectively) showed significant differences (P <0.05) than the Control group (55.9 ± 8.7%). The results obtained in this study showed that the use of deep freezer at -80°C for freezing and storing canine sperm has potential use in veterinary medicine as an alternative to the use of N2L.

Keywords : Canine; Sperm freezing; Ultrafreezer at -80C.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License