SciELO - Scientific Electronic Library Online

 
vol.31 número1Determinación del Volumen del Ventrículo Lateral y del Cerebro en Niños Utilizando el Método Estereológico Mediante RNMPerfil Somatotípico del Futbolista Profesional Chileno índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

Compartir


International Journal of Morphology

versión On-line ISSN 0717-9502

Resumen

SALINAS, Paulo; SANCHEZ, Raúl  y  RISOPATRON, Jennie. Cryopreservation of Canine Spermatozoa at -80C. Int. J. Morphol. [online]. 2013, vol.31, n.1, pp.217-224. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022013000100036.

The freezing and storage in liquid nitrogen (LN2) is the technique used most for the preservation of canine and other domestic species semen, for long periods of time. This study was designed to validate the use of deep freezer at -80C to freeze and store canine semen in straws. Three protocols were tested (n = 10): Control: sperm frozen in N2L and stored; Exp 1: sperm frozen and stored -80°C, and Exp.2: semen frozen at -80 ° C and stored in N2L. Post-thawing was assessed by flow cytometry and the viability of plasma membrane integrity (SYBR-14/PI), mitochondrial membrane potential (YDm, JC-1), acrosome membrane integrity (PSA / FITC-PI), translocation of phosphatidylserine (Annexin -V-FITC/PI) and DNA fragmentation (TUNEL). No significant differences (P <0.05) between the Control, Exp.2 and Exp.1groups regarding: intact plasma membrane integrity (42.2 ± 5.3, 35.57 ± 10.3 and 40.76 ± 12.1, respectively), YDm normal (54.7 ± 20.6, 44.4 ± 15.8 and 43.4 ± 15.3, respectively), intact acrosomal membrane integrity (42.9 ± 11 , 1, 53.2 ± 15.8 and 48.7 ± 20.1, respectively) and fragmented DNA (0.87 ± 0.41, 0.75 ± 0.16, 0.79 ± 0.27, respectively). However, the average motility of the post-thawing of Exp.1 and Exp.2 groups (37.0 ± 4.4% 36.8 ± 4.1% respectively) showed significant differences (P <0.05) than the Control group (55.9 ± 8.7%). The results obtained in this study showed that the use of deep freezer at -80°C for freezing and storing canine sperm has potential use in veterinary medicine as an alternative to the use of N2L.

Palabras clave : Canine; Sperm freezing; Ultrafreezer at -80C.

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons