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International Journal of Morphology

On-line version ISSN 0717-9502

Abstract

LIU, Chongbin et al. A Research of Peripheral Blood Cells Annually in Bufo Bufo gargarizans. Int. J. Morphol. [online]. 2013, vol.31, n.4, pp.1282-1288. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022013000400022.

The present paper deals with a histological study of the blood cells of Bufo Bufo gargarizans in different months: January, March, May, July and October. The methods used are by routine blood smear in Wright stain and observation in vivo. We found that in smears and in vivo two main types of cells of the red cells: mitotic as well as amitotic. While amitotic occurs all the year round, particularly in July, mitosis so far had been seen only in July. It is also found that there are plenty of neutrophils in the blood cells of Bufo Bufo gargarizans, furthermore, the nuclei of these cells are polymorphic, especially in January and March. Meanwhile, the concentration of red cells was lowest in May and highest in January; The concentration of white blood cells was highest in October and lowest in March; As to granulocytes, eosinophils in July and October had higher proportion, while neutrophils and basophils in July; in agranulocytes, mononuclear cells reached the highest value in March, lowest in January, lymphocytes and the maximum value appeared in May, the lowest value appeared in July. Morphological changes of thrombocytes were not obvious.

Keywords : Bufo Bufo gargarizans; Blood cells; Annual changes.

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