SciELO - Scientific Electronic Library Online

 
vol.32 issue2The Recurrent Laryngeal Cardiac Nerve in FetusesMorphological Alterations in Mouse Placenta Induced by Diazepam author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


International Journal of Morphology

On-line version ISSN 0717-9502

Abstract

RAZI, Elahe Mirarab; GHAFARI, Soraya; HOJATI, Vida  and  GOLALIPOUR, Mohammad Jafar. Effect of Gestational Diabetes on Neuronal Cells in Rat Cerebellum in Early Postnatal Life. Int. J. Morphol. [online]. 2014, vol.32, n.2, pp.420-425. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022014000200006.

Previous study has shown the adverse effects of gestational diabetes on hippocampal neuronal density in animal model. This study was conducted to determine the effect of gestational diabetes on rat cerebellum in early postnatal life. In this experimental study, 10 dams randomly allocated into control and diabetic groups on day 1 of gestation. Five dams in diabetic group were administered 40 mg/kg/BW (intraperitoneally) of streptozotocin and control animals received normal saline. Six offspring of each gestational diabetes mellitus and controls were randomly selected at day 7 of postnatal life. Offspring were sacrificed and coronal sections were taken from the cerebellum and stained with cresyl violet. The number of Purkinje and granular cells and thickness of layers of cerebellum were evaluated by quantitative computer-assisted morphometric method. The Purkinje cells density at apex and depth of cerebellar lobules in the experimental group (14.40±0.7, 14.86±0.6) significantly reduced in comparison with the control group (16.72±0.3, 17.85±0.7) (P<0.05). The granular cell density at apex and depth of cerebellar lobules in the experimental group (23.94±0.6, 22.81±0.5) significantly reduced in comparison with the control group (29.20±0.8, 28.1±0.8) (P<0.05). The thickness of the Purkinje and internal granular and molecular layers at apex and depth of cerebellar cortex significantly reduced in diabetics group compared to controls (P<0.05). This study revealed that gestational diabetes induces loss of number and size of the Purkinje cells and the granular cells and reduction of thickness of the Purkinje and internal granular layer of the cerebellar cortex in neonatal mice.

Keywords : Gestational diabetes; Cerebellum; Purkinje cell; Granular cell; Rat.

        · abstract in Spanish     · text in English     · English ( pdf )