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International Journal of Morphology

On-line version ISSN 0717-9502


BRIZUELA CORDERO, Claudia; SAINT JEAN, Nicole  and  INOSTROZA SILVA, Carolina. Biocompatibility Of Mesenchymal Stem Cells Derived From Human Gingival Tissue In Culture With A Scaffold Of A Synthetic Polymer Open Porosity Polylactic Acid Polymer (OPLA). Int. J. Morphol. [online]. 2014, vol.32, n.3, pp.767-772. ISSN 0717-9502.

Tissue engineering arises as the ideal treatment for tissue regeneration with the use of scaffolds, stem cells and growth factors. Stem cells derived from gingival tissue present benefits in its objection. 3D OPLA allow high cell density cultures. The objective of this study was to evaluate the biocompatibility of gingival stem cells in OPLA. Cells were obtained from gingival tissue and were characterized phenotypically and functionally. The biocompatibility was evaluated through cell proliferation, viability test with trypan blue and cell differentiation to chondrogenic and osteogenic lineage. Recultivation of the construct was used to evaluate transportability. Cells inside OPLA were visualized by stained sections with H&E. Mesenchymal stem cells proliferated in OPLA, 80% confluence at the fourth week. Cell viability in OPLA was 83.32%. In recultivation, cells start proliferating in a week. OPLA allows cell differentiation to chondrogenic and osteogenic lineage. Cells were observed within OPLA. In conclusion OPLA allows proliferation, viability and cell differentiation. OPLA could be used as scaffolds for cells in tissue engineering.

Keywords : Mesenchymal stem cells; Gingival connective tissue; Giocompatibility.

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