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International Journal of Morphology

On-line version ISSN 0717-9502

Abstract

CALVO, J et al. Collection, Evaluation and Freezing of Sperm Antilope Adult (Addax nasomaculatus) of the Lecocq Zoo-Park in Montevideo-Uruguay. Int. J. Morphol. [online]. 2016, vol.34, n.1, pp.276-279. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022016000100039.

For almost 50 years sperm cryopreservation has been an important reproductive biotechnology that promotes the conservation of male germplasm. While today automated portable electronic systems have been developed, many technicians continue to use the conventional method of freezing for its practicality, low cost and efficiency. In this work we present preliminary data using standard protocols for assessing reproductive males of other species, about evaluation and freezing of adult antelope (Addax nasomaculatus) male gamete. Semen from three adult male antelope was obtained; we registered volume, color, motility, vigor, vitality, concentration and morphology. The semen was frozen by the conventional method and preserved under -196 C nitrogen. Males responded satisfactorily to the method of sedation and extraction of semen. The evaluation of fresh and frozen semen showed acceptable preliminary data that validate the methodology and encourage them to continue working with this species because of their status of animals in captivity and in danger of extinction.

Keywords : Sperm; Cryopreservation; Antelope.

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