SciELO - Scientific Electronic Library Online

 
vol.35 número4Circunferencia Occipito-Frontal como Predicción de la Estatura en un Grupo de Adultos de Sri LankaEfectos del Ranelato de Estroncio en el Aumento del Rebforde Alveolar índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


International Journal of Morphology

versión On-line ISSN 0717-9502

Resumen

CHAICHUN, Amnart et al. Localization and Identification of Tyrosine Phosphorylated Proteins in Adult Sprague-Dawley Rat Testis. Int. J. Morphol. [online]. 2017, vol.35, n.4, pp.1322-1327. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022017000401322.

Spermatogenesis is a major process in testis occurring from puberty through life span of males. The tyrosine phosphorylation is assumed to play roles in spermatogenesis because this process is important for cell proliferations, divisions, and differentiations. However, the localizations and identifications of phosphorylated proteins in testicular tissue of adult male rats are still unclear. Therefore, this study attempted to immuno-localize and identify such proteins in testicular tissues of Sprague-Dawley rats. The monoclonal anti-phosphotyrosine (clone 4G10) was used to probe tyrosine phosphorylated proteins and also to examine the expression of such proteins using immuno-Western blotting in rat testis. The result showed that positive reactivity of tyrosine phosphorylated proteins was clearly observed in interstitial endocrine cells (Leydig cells), sustentocytes (Sertoli cells), spermatogonia, spermatocytes, and spermatids (round and elongated), respectively. The expressions of testicular tyrosine phosphorylated proteins were 200, 131, 93, 70, 60, and 48 kDas, respectively. In conclusion, testicular tyrosine phosphorylated proteins were localized in both germinal epithelium and interstitial endocrine cells of adult Sprague-Dawley rats.

Palabras clave : Localization; Identification; Tyrosine phosphorylated proteins; Testis; Rats.

        · resumen en Español     · texto en Inglés     · Inglés ( pdf )