SciELO - Scientific Electronic Library Online

 
vol.36 issue2Morphological Changes of Skeletal Class III Malocclusion in Mixed Dentition with Protraction Combined ActivitiesEpoxy Sheet Plastination on a Rabbit Head - New Faster Protocol with Biodur® E12/E1 author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


International Journal of Morphology

On-line version ISSN 0717-9502

Abstract

ELYASI, Leila; JAHANSHAHI, Mehrdad; GHAZVINI, Hamed  and  NIKMAHZAR, Emsehgol. The Protective Effects of Citrus aurantium Flower Extract against 6-Hydroxydopamine-Mediated Cell Damage in Human Neuroblastoma SH-SY5Y Cells. Int. J. Morphol. [online]. 2018, vol.36, n.2, pp.435-440. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022018000200435.

Parkinson’s disease (PD) is described as a neurological condition, resulting from continuous degeneration of dopaminergic neurons. Currently, most treatments for neurodegenerative diseases are palliative. In traditional Iranian medicine, Citrus aurantium flower extract is used to treat some neural diseases, such as sleep disorders and anxiety. The tendency towards the use of medicinal herbs for the treatment of diseases (eg, seizure) is growing. Accordingly, we evaluated the antioxidant effects of C. aurantium flowers and analyzed their protective effects against 6-hydroxydopamine (6-OHDA)-mediated oxidative stress. In this study, 150 mM of 6-OHDA was used to induce cellular damage. Also, MTT assay was performed to analyze cellular viability. Fluorescence spectrophotometry was performed to measure the intracellular reactive oxygen species (ROS) and calcium levels. Based on the findings, 6-OHDA could reduce cell viability. We also analyzed the effects of C. aurantium against neurotoxicity. The intracellular levels of ROS and calcium greatly improved in cells exposed to 6-OHDA. SH-SY5Y cell incubation with C. aurantium (400 and 600 mg/mL) induced protective effects and decreased the biochemical markers of cell apoptosis. According to the findings, C. aurantium showed protective effects against neurotoxicity, caused by 6-OHDA; these protective properties were accompanied by antiapoptotic features. According to the findings, it seems that hydromethanolic C. aurantium extract can be used to prevent seizures.

Keywords : Citrus aurantium; Parkinson’s disease; 6-Hydroxydopamine; Apoptosis; Antioxidant activity.

        · abstract in Spanish     · text in English     · English ( pdf )