SciELO - Scientific Electronic Library Online

 
vol.36 número3La Estimulación Táctil/Kinestésica Revierte los Efectos del Estrés Prenatal en la Región CA3 del Hipocampo en Ratas Hembras: Estudio EstereológicoCaracterización Morfológica y Morfométrica del Tubérculo Esfenoidal índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


International Journal of Morphology

versión On-line ISSN 0717-9502

Resumen

LUCENA, Eudes Euler de Souza et al. Expansion and Phenotypic Changes of Mouse Bone Marrow Mesenchymal Cells Cultured with FGF-2 and Facial Nerve-Conditioned Medium. Int. J. Morphol. [online]. 2018, vol.36, n.3, pp.1049-1056. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022018000301049.

Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation.

Palabras clave : Mesenchymal stem cells; Facial nerve injury; Fibroblast growth factor 2; Conditioned medium; Plasticity; Trans-differentiation.

        · resumen en Español     · texto en Inglés     · Inglés ( pdf )