SciELO - Scientific Electronic Library Online

 
vol.36 número3Efectos de la Exposición de la Dieta Alta en Grasa Materna y Pos Lactancia en la Función del Hipocampo y la MorfologíaVariaciones Anatómicas del Foramen Mental: Un Estudio Transversal Retrospectivo índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


International Journal of Morphology

versión On-line ISSN 0717-9502

Resumen

SALINAS, Paulo; SANHUEZA, Jorge  y  SANDOVAL, Carlos. Color-Based Segmentation vs. Stereology: A Simple Comparison Between Two Semi-Automated Methods of Image Analysis for the Quantification of Collagen. Int. J. Morphol. [online]. 2018, vol.36, n.3, pp.1118-1123. ISSN 0717-9502.  http://dx.doi.org/10.4067/S0717-95022018000301118.

Image processing techniques are being widely developed for helping specialists in analysis of histological images and its application is especially useful in obtaining numerical data for the realization of the subsequent statistical analysis. The use of these methods makes the histological analysis of experts more objective and less time-consuming. In this paper we evaluate how well the quantitative methods - color-based image segmentation and stereology - agree on average, and how well they agree for the individuals when they are used to quantify type I and III collagen. Digital images of sections of Salmo salar jaws (5 µm, SiriusRed staining) were analyzed. Collagen quantification was performed by two methods in the same group of images: i) Color Based-Segmentation (K-means algorithm; pixel cluster; ImageJ32 v1.51p) and ii) Stereology (VV; M36; STEPanizer Stereological Tools). They were evaluated 200 images per group. The difference between groups and concordance was analyzed using t-Student (p<0.05) and Blant Altman Comparison Method, respectively. The data analysis of average and individual assessments showed that there is concordance between two methods. In conclusion, stereology and color-based image segmentation are powerful tools which quantify collagen in histological sections.

Palabras clave : Collagen; Stereology; Segmentation; Image Analysis; Histology.

        · resumen en Español     · texto en Inglés     · Inglés ( pdf )