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vol.52 número2POLYPHOSPHAZENES WITH P(DIOXY-BIARYL) CYCLES IN THE REPEATING UNITS: A REVIEWON THE METALLICITY OF SOME CARBON NANOTUBES índice de autoresíndice de assuntospesquisa de artigos
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Journal of the Chilean Chemical Society

versão On-line ISSN 0717-9707

Resumo

ABUIN, E; ASPEE, A; LISSI, E  e  LEON, L. BINDING OF ROSE BENGAL TO BOVINE SERUM ALBUMIN. J. Chil. Chem. Soc. [online]. 2007, vol.52, n.2, pp.1196-1197. ISSN 0717-9707.  http://dx.doi.org/10.4067/S0717-97072007000200017.

The association of Rose Bengal (RB) with bovine serum albumin (BSA) was investigated by absorbance spectroscopy. The binding constant was determined from the effect observed in the absorbance of RB at 548 nm upon addition of the protein according with the Benesi-Hildebrand treatment. Results were obtained in phosphate buffer at pH = 7.0. The effect of the salinity of the buffer and the sensitivity of the binding constant to the presence of urea were also studied. The results obtained allow to conclude that the binding of RB to BSA is dominated by hydrophobic effects

Palavras-chave : Rose Bengal; bovine serum albumin.

        · texto em Inglês

 

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