SciELO - Scientific Electronic Library Online

 
vol.53 issue2SECONDARY METABOLITES FROM PEPEROMIA SUI author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Journal of the Chilean Chemical Society

On-line version ISSN 0717-9707

Abstract

TORAL, M. INÉS et al. SIMULTANEOUS DETERMINATION OF ACETAMINOPHEN AND TRAMADOL BY SECOND DERIVATIVE SPECTROPHOTOMETRY. J. Chil. Chem. Soc. [online]. 2008, vol.53, n.2, pp.1543-1547. ISSN 0717-9707.  http://dx.doi.org/10.4067/S0717-97072008000200022.

A rapid method for the simultaneous determination of acetaminophen (A) and tramadol (T) by second derivative spectrophotometric has been developed. From a solvent effect studies and the spectral behaviours of A and T, ethanol was selected as solvent. For a Δλ value of 210 nm a smoothing factor of 8,000 and scale factor of 1,000,000 were selected, because in these conditions the signal/noise ratio was favoured in order to avoid error. In these conditions also it is possible the simultaneous determination of A/T in a molar relation of 17/1 contained in pharmaceutical formulations. At 285.7 nm the second derivative value is T concentration dependent, corresponding to zero-crossing point of A. On the other hand, T does not absorb between 296.0 to 400.0 nm, thus 308.0 nm was selected for A determination by graphic method. The determination ranges for A and T were 8.1x10-7 - 5 1x10-5 mol/L and 3.4 x10-7 - 5.0 x10-5 mol/L, respectively and can be determined with good precision and accuracy, without previous separation. A study of interferents, was carried out by using the excipients Zafin® tablet. The recoveries were 97.4 ± 2.0% and 100.6 ± 1.7% for A and T, respectively. This tablet containing both drugs was assayed using the methods developed, showing a good accuracy and precision.

Keywords : Acetaminophen; Tramadol; Simultaneous Determination; Derivative Spectrophotometry; Pharmaceutical Formulation.

        · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License