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Journal of the Chilean Chemical Society

On-line version ISSN 0717-9707

Abstract

SURENDRA BABU, M.S; KRISHNA, PITCHIKA.G; HUSSAIN REDDY, K  and  PHILIP, G.H. INVESTIGATIONS ON NUCLEASE ACTIVITY OF TRANS MIXED LIGAND- COPPER(II) COMPLEXES WITH ORTHO SUBSTITUTED AROMATIC OXIMES AND HETEROCYCLIC BASES. J. Chil. Chem. Soc. [online]. 2009, vol.54, n.4, pp.339-344. ISSN 0717-9707.  http://dx.doi.org/10.4067/S0717-97072009000400003.

Mixed ligand complexes of copper(II) with salicyladoxime (SAO), 2-hydroxy acetophenone oxime (HAO) as primary ligands and pyridine( Py), imidazole (Im) as secondary ligands have been synthesized and characterized by molar conductivity, magnetic moments, electronic, IR and ESR spectral data. Cyclic voltammetric studies show quasi-reversible reduction attributable to Cu2+/ Cu+. The binding interactions between metal complexes and calf thymus DNA have been investigated by using UV -Visible titrations and cyclic voltammetry studies. The cleavage activity of complexes was carried out on double-stranded pBR322 circular plasmid DNA by using gel electrophoresis. All complexes show increased nuclease activity in the presence of oxidant (H202). The nuclease activity of mixed ligand complexes are compared with that of the parent copper(II) complexes. Controlled experiments suggest that the complexes cleave DNA predominantly via an oxidative mechanism.

Keywords : Copper(II) complexes; mixed ligand complexes; aromatic oximes; DNA binding; oxidative cleavage.

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