SciELO - Scientific Electronic Library Online

 
vol.56 número4SYNTHESIS, CHARACTERIZATION AND THERMAL STUDIES OF WHOLLY AROMATIC POLY(IMIDE-AMIDES) CONTAINING SI AND/OR GE IN THE MAIN CHAINPREPARATION AND CHARACTERIZATION OF THE EMAMECTIN BENZOATE/HYDROXYPROPYL-B-CYCLODEXTRIN INCLUSION COMPLEX índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Journal of the Chilean Chemical Society

versão On-line ISSN 0717-9707

Resumo

ABUIN, ELSA; LISSI, EDUARDO  e  BRIDI, RAQUEL. KINETICS OF P - NITROPHENYL ACETATE HYDROLYSIS CATALYZED BY α- CHYMOTRYPSIN IN PRESENCE OF POLYETHYLENE GLYCOL. J. Chil. Chem. Soc. [online]. 2011, vol.56, n.4, pp.948-950. ISSN 0717-9707.  http://dx.doi.org/10.4067/S0717-97072011000400028.

The effect of poly (ethyleneglycol) (PEG) addition on the rate of p-nitrophenyl acetate (PNPA) catalyzed by a- chymotrypsin has been measured in TRIS buffer at pH = 7.4. The presence of PEG (up to 30 % p/v) leads to an increase in the rate of the process. The effect of PEG is evident both, in the pre-steady state and steady state stages of the process. The reaction follows a Michaelis-Menten mechanism under all the conditions employed. The Michaelis constant slightly decreases and the catalytic rate constant slightly increases with PEG concentration over all the polymer concentration range employed (up to 30%). The decrease in the Michaelis constant suggests that the effect of the polymer is due to crowding.

        · texto em Inglês     · Inglês ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons