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Journal of the Chilean Chemical Society

versión On-line ISSN 0717-9707

Resumen

BRAVO, FELIPE et al. VALIDATION OF A METHOD TO DETECT COCAINE AND BENZOYLECGONINE IN HUMAN WHOLE BLOOD BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND APPLICATION IN BODY PACKERS AND STUFFERS CASES IN THE NORTH OF CHILE. J. Chil. Chem. Soc. [online]. 2012, vol.57, n.3, pp.1253-1255. ISSN 0717-9707.  http://dx.doi.org/10.4067/S0717-97072012000300012.

The present paper describes a method for the simultaneous determination of cocaine and benzoylecgonine in human whole blood. The drugs were extracted with phosphate buffer at pH 6, followed by solid-phase extraction and quantification by GC/MS with electron impact ionization using helium as carrier gas. Quantification was performed using cocaine-d3 as internal standard in selected ion monitoring mode. The method is very simple, rapid and sensitive. The specificity, linearity, intra- and inter-assay precision and accuracy, and extraction recovery were fully evaluated. The limits of detection were 3.6 ng/mL for cocaine and 6.8 ng/mL for benzoylecgonine. The method was applied to blood samples removed at autopsy from body packers and stuffers cases in the I - IV and XV regions of Chile during 2008-2010.

Palabras clave : Benzoylecgonine; cocaine; gas chromatography; mass selective detector; solid-phase extraction; body packers-stuffers.

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