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vol.61 issue1CHEMICAL CHARACTERIZATION OF SUB-BITUMINOUS COAL FROM THE ARAUCO PROVINCE - CHILEMOLYBDENUM TRIOXIDE THIN FILMS DOPED WITH GOLD NANOPARTICLES GROWN BY A SEQUENTIAL METHODOLOGY: PHOTOCHEMICAL METAL-ORGANIC DEPOSITION (PMOD) AND DC-MAGNETRON SPUTTERING author indexsubject indexarticles search
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Journal of the Chilean Chemical Society

On-line version ISSN 0717-9707

Abstract

OZDEMIR, SERKAN  and  BICER, ENDER. TEMPERATURE EFFECT ON BINDING AFFINITY AND STOICHIOMETRY BETWEEN SOME STEROIDS AND HUMAN SERUM ALBUMIN. J. Chil. Chem. Soc. [online]. 2016, vol.61, n.1, pp.2809-2815. ISSN 0717-9707.  http://dx.doi.org/10.4067/S0717-97072016000100013.

The temperature dependence of the interactions between some steroids (fusidic acid, ouabain and ethynodioldiacetate) and human serum albumin (HSA) at physiological pH (7.4) were studied systematically by square-wave voltammetry. Also, these interactions were proven by means of UV-Vis. absorptionspectroscopy and FT-IR spectroscopy techniques. The interaction parameters (binding constants and stoichiometries) at temperature range of 297.5-312.5 K weredetermined from electrochemical data. It is worthy that the temperature has played a positive role on the interactions of fusidic acid with HSA; however it hasnegative effective on the interaction parameters of HSA with ouabain or ethynodioldiacetate.

Keywords : steroids; human serum albumin; binding affinity; temperature effect; voltammetry.

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