SciELO - Scientific Electronic Library Online

 
vol.61 issue3FORMATION OF 2D SHEETS THROUGH H…H INTERACTION IN THE CRYSTAL PACKING OF 1,2-BIS(3-BROMOBENZYLIDENE)HYDRAZNEINTELLIGENT MULTIVARIATE MODEL FOR THE OPTICAL DETECTION OF TOTAL ORGANIC CARBON author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Journal of the Chilean Chemical Society

On-line version ISSN 0717-9707

Abstract

AHUMADA, M; CALDERON, C  and  LISSIE, A. TEMPERATURE DEPENDENCE OF BILAYER PROPERTIES IN LIPOSOMES AND THE USE OF FLUORESCENT PROBES AS A TOOL TO ELUCIDATE THE PERMEATION MECHANISM OF HYDROPHILIC SOLUTES. J. Chil. Chem. Soc. [online]. 2016, vol.61, n.3, pp.3052-3054. ISSN 0717-9707.  http://dx.doi.org/10.4067/S0717-97072016000300009.

Solute transport across lipidic membranes is a fundamental process for both living organisms and drug delivery. In order to establish the mechanism of solute passive transport through lipidic membranes, we determined the effect of temperature upon the rate of glucose transport (as hydrophilic solute model) through dipalmitoylphosphatidylcholine large unilamellar liposomes and compare the results to those obtained for a variety of fluorescent probes (pyrene, PRODAN, diphenylhexatriene, diphenylhexatriene-TMA). All these probes, independent of their localization in the liposomes, report a monotonous change in the micro-properties sensed with temperature, with a maximum rate of change at the main transition temperature of the bilayer. These results contrast with those obtained for the rate of glucose influx, were it is observed a clear maximum rate of intake at the transition temperature. These contrasting results imply that the microviscosity (common factor for used probes) is not the property of the bilayer that controls the rate off solute transport which, at least in the vicinity of the main transition temperature, is regulated by formation of transient pores, particularly at the transition temperature (Tm). This would indicate that, at least near Tm, glucose and other hydrophilic solutes diffuses through transient pores formed in the bilayer. Interestingly, the presence of these pores does not affect the fluorescence characteristics of the probes.

Keywords : liposome; permeation; glucose; glucose oxidase; fluorescent probe.

        · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License