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Información tecnológica

versión On-line ISSN 0718-0764

Resumen

DIAZ, Luis S; PADILLA, Claudia  y  SEPULVEDA, Carolina. Identification of the Main Pigment Present in the Skin of the Purple Passion Fruit (Passiflora edulis). Inf. tecnol. [online]. 2006, vol.17, n.6, pp.75-84. ISSN 0718-0764.  http://dx.doi.org/10.4067/S0718-07642006000600013.

The objective of the present study was the identification of the main pigment present in the skin of purple passion fruit (Passiflora edulis). Skins of purple passion fruit were extracted with 0.01%HCl in methanol. The extract was analyzed by chromatography, with subsequent determination of the Rf value for the pigment using paper and silica gel chromatography The pigment was submitted to acid hydrolysis in 2M HCl to separate the sugar from the anthocyanidin. The identification of the anthocyanidin was carried out indirectly through alkaline hydrolysis with 20% Ba(OH)2 . The sugar was determined by comparing its Rf value on Whatman N° 1 paper with data from the literature. The pigment was identified by applying the expressions which relate: Dlmax. anthocyano - lmax. anthocyanidin, and that which relates DA/A = (A 440/Amax. anthocyanidin) x 100. It is concluded that the pigment present in the skin of the purple passion fruit is malvidin 3-monoglucoside

Palabras clave : passion fruit; pigment identification; absorption properties.

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