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Información tecnológica

On-line version ISSN 0718-0764


GOMEZ, Leidy J; FIGUEROA, Omar A  and  ZAPATA, José E. Antioxidant Activity of Bovine Plasma Enzymatic Hydrolysates Obtained by Effect of Alcalase® 2.4 L. Inf. tecnol. [online]. 2013, vol.24, n.1, pp.33-42. ISSN 0718-0764.

The antioxidant activity of bovine plasma hydrolysates (BPH) obtained with Alcalase 2.4 L at different hydrolysis degrees was evaluated. The hydrolysates were fractioned through ultrafiltration membranes and were purified by ion-exchange chromatography, with subsequent reverse-phase high performance liquid chromatography. The results showed that the hydrolysates of bovine plasma obtained under the established hydrolysis conditions, had strong scavenging ability on 2,2-azino bis-(3-ethylbenzothiazoline)-6-sulfonic acid free radicals and a great reduction power compared with proteins of non-hydrolyzed plasma. Additionally, it was found that the antioxidant activity increases as function of the hydrolysis degree, and that this activity remains after in-vitro digestion. The processes described allow obtaining a peptid with antioxidant activities similar to that of conventional commercial antioxidants.

Keywords : bovine plasma; antioxidant activity; antioxidant peptides; alcalase.

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