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Información tecnológica

versión On-line ISSN 0718-0764

Resumen

VASQUEZ, Neil A; TORRES, Viviana  y  ROJANO, Benjamín A. EFFECT OF ASCORBIC ACID DURING BOVINE OOCYTES IN VITRO MATURATION ON REACTIVE OXYGEN SPECIES (ROS) PRODUCTION AND EMBRYO DEVELOPMENTAL COMPETENCE. Inf. tecnol. [online]. 2014, vol.25, n.2, pp.141-150. ISSN 0718-0764.  http://dx.doi.org/10.4067/S0718-07642014000200016.

In this study the effects of fetal calf serum (FCS), bovine serum albumin (BSA), and the antioxidants alpha tocopherol (AT) and ascorbic acid (AA), on the production of reactive oxygen species (ROS) in culture media, in vitro matured (IVM) bovine oocytes and the rate of embryo development were evaluated. The culture conditions were as follows: incubation at 38.5°C, with 5%CO2 and 95% of humidity. The results showed higher production of ROS in culture media supplemented with FCS (p<0.05), while ROS production was similar in culture media containing BSA and FBS-BSA (p>0.05).AA and AT showed antioxidant effect on culture media containing serum and in success rate of IVM oocytes, but AA only improved the developmental competence of oocytes. This result provides evidence on the importance of reducing ROS production during IVM for improvement of in vitro bovine embryo production.

Palabras clave : antioxidants; bovine serum; reactive oxygen species; serum albumin.

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