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Información tecnológica

On-line version ISSN 0718-0764

Abstract

GALVIS, Fabián  and  CARRILLO, Marlen. Identification and Characterization Molecular of Burkholderia glumae: Causal Agent of Bacterial Panicle Blight in Rice. Inf. tecnol. [online]. 2015, vol.26, n.3, pp.33-40. ISSN 0718-0764.  http://dx.doi.org/10.4067/S0718-07642015000300006.

The aim of this study was to identify and molecularly characterize isolates of Burkholderia glumae from rice plants with evidence of putrescence. Burkholderia glumae is the causal agent of bacterial blight of panicle rice, with a high incidence causing considerable losses in crop production in Colombia. Since its identification is difficult, it is important to implement an evaluation system to quickly and accurately identify this pathogen. Sixty four isolates were obtained, 41 of which were identified by polymerase chain reaction (PCR) as Burkholderia glumae. The molecular characterization with MB1 and BOX-PCR markers showed genetic variability in most isolates, but allowed the grouping according to its geographical origin. The high genetic diversity suggests a high degree of variability within individuals of this species.

Keywords : Burkholderia glumae; bacterial panicle; rice cultivation; PCR.

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