Abstract

VANEGAS, Diana M  and  RAMIREZ, Margarita E. Correlation of Pseudomonas fluorescens Growth in the Production of Medium Chain Length Polyhydroxy-alkanoates (PHA_MCL) by Gompertz, Logistic and Baranyi Primary Models. Inf. tecnol. [online]. 2016, vol.27, n.2, pp.87-96. ISSN 0718-0764.  http://dx.doi.org/10.4067/S0718-07642016000200011.

In this work, models to correlate the growth of Pseudomonas fluorescens (a gram negative rod shaped bacterium) in the production of medium chain length polyhydroxy-alkanoates (PHA_MCL) are analyzed. Sugarcane molasses and glucose were evaluated as carbon source by the cellular growth and production of polyhydroxyalcanoates. It was found that the molasses at concentrations lower than 60 g/L and 10 g/L of reducing sugars promotes the polihydroxialcanoate production of medium length chain. Prediction of Pseudomonas fluorescens growth was done using the primary kinetic models of Gompertz, Logistic and Baranyi. Growing kinetic parameters, such as latency (λ), maximum specific growth rate (μ_max), and maximum cell concentration (y_max), were determined. Gompertz and Baranyi models showed the best fit to the experimental results, exhibiting some discrepancies in latency values. However, the three models show a correlation value R² higher than 0.98.

Keywords : Pseudomonas fluorescens; Growth kinetic; secondary metabolites; molasses; medium chain length polyhydroxyalkanoates; PHA_MCL.

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