SciELO - Scientific Electronic Library Online

vol.39 issue1Management, production, and nutritional characteristics of cup-plant (Silphium perfoliatum) in temperate climates of southern ChileCold storage effects on oxidative stress of Red Globe table grape rachises author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Ciencia e investigación agraria

On-line version ISSN 0718-1620


SALAZAR-GONZALEZ, Claudia; VERGARA-BALDERAS, Fidel T; ORTEGA-REGULES, Ana E  and  GUERRERO-BELTRAN, José Á. Antioxidant properties and color of Hibiscus sabdariffa extracts. Cienc. Inv. Agr. [online]. 2012, vol.39, n.1, pp.79-90. ISSN 0718-1620.

Liquid extracts of dried Roselle (Hibiscus sabdariffa) calyces obtained using ethanol:water (50:50 and 70:30%, v/v), water, ethanol:1.5 N HCl (85:25%, v/v), and ethanol (96%) to evaluate selected antioxidant characteristics (phenolic compounds and antioxidant capacity), color parameters (L, a, and b), and anthocyanins (delphinidin-3-O-sambubioside, delphinidin-3-O-glucoside (myrtillin), and cyanidin-3-O-sambubioside) by high performance liquid chromatography (HPLC). The total monomeric anthocyanin content was also assessed using the differential pH technique. The phenolic compound content ranged from 1,067±22 (in ethanol) to 2,649±96 (in ethanol:water 70:30%) mg of gallic acid100 g-1 of dehydrated calyces, and the antioxidant capacity ranged from 3.11±0.50 (in ethanol) to 8.0±0.2 mmoles of trolox100 g-1 of calyces. The total monomeric anthocyanin content (209±21 mg100 g-1) was similar to that obtained using the HPLC technique (215±31 mg100 g-1) when extracting the calyces with the 50:50% (v/v) ethanol:water solution. The deepest red-purple (Hue = 62.50±0.34) colored solution was observed when extracting the calyces with ethanol. The antioxidant and color properties of Roselle calyces make the extracts ideal for use in foods as a natural extract, concentrate, or powder.

Keywords : Anthocyanins; antioxidant capacity; cyanidin-3-O-sambubioside; delphinidin-3-O-sambubioside; delphinidin-3-O-glucoside; myrtillin; Hibiscus sabdariffa; Roselle calyces.

        · abstract in Spanish     · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License