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Ciencia e investigación agraria

On-line version ISSN 0718-1620

Abstract

PAREDES, Kelly et al. In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile. Cienc. Inv. Agr. [online]. 2014, vol.41, n.2, pp.207-214. ISSN 0718-1620.  http://dx.doi.org/10.4067/S0718-16202014000200007.

K. Paredes, C. Delaveau, P. Carrasco, C. Baeza, Freddy Mora, and M.E. Uribe. 2014. In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile. Cien. Inv. Agr. 41(2): 207-214. Critically endangered, Traubia modesta is endemic to Chile and belongs to the family Amaryllidaceae. In this research, a propagation protocol was developed for the in vitro cultivation of vegetative organs for this species. The twin scale explants were cultured in a Murashige and Skoog (MS) medium, supplemented with the growth regulators naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) in different concentrations. Plant preservative mixture (PPM) and polyvinyl pyrrolidone (PVP) were used as a biocide and an antioxidant, respectively. The results showed high contamination of the bulbs. There were no significant differences between the treatments with plant growth regulators. A multiplication rate of 1.3 - 2.2 for bulbils was achieved, with an average of 28 bulbils per mother bulb. The natural regeneration rate is approximately 1 - 4 bulbils per mother bulb once a year.

Keywords : Bulbing; critically endangered species; In vitro propagation; Traubia modesta; twin scales.

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