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Revista de biología marina y oceanografía

On-line version ISSN 0718-1957


RODRIGUEZ-IBARRA, L. Estela et al. Effect of formalin, acriflavine and glutaraldehyde on disinfecting and hatching of the bullseye puffer fish Sphoeroides annulatus. Rev. biol. mar. oceanogr. [online]. 2011, vol.46, n.1, pp.59-65. ISSN 0718-1957.

Three prophylactic treatments using three dosages each were used as surface disinfectants in bullseye puffer Sphoeroides annulatus eggs to study their effect on bacterial load reduction and hatching rate. Eggs were treated with proteolytic enzyme to remove stickiness. Tested treatments were: acriflavine (A) at a concentration of 2.5, 5 and 10 ppm for 1 min; formalin (F) at 10, 50 and 100 ppm for 30 min and glutaraldehyde (G) at 50, 100 and 200 ppm for 5 min, as well as a control group with no treatment (C), using three replicates per treatment. After disinfection, an egg sample from each replicate was inoculated in duplicate on TCBS and marine agar culture media to assess bacterial growth (CFU mL-1). The eggs were incubated and their hatching rate determined. Culture media tested negative for Vibrio spp. in all treatments. However, the growth of heterotrophic bacteria was present in all experimental groups; G-100 showed a significant reduction in CFU (4.00 ± 2.8 x 102 mL-1), whereas A-2.5 and F-50 had significantly higher CFU values (3.45 ± 0.4 x 104 and 5.73 ± 0.3 x 104 mL-1 respectively). By not disinfecting the eggs, a higher hatching rate was obtained (96.1 ± 6.2%), however relatively high CFU counts (1.22 ± 0.1 x 104 mL-1) were observed in this treatment, while the lowest hatching rate was found in eggs disinfected with A-5 (12.3 ± 6.3%). Thus, the use of stickiness removal procedures prior to incubation of bullseye puffer eggs seems to be enough to produce good hatching rates.

Keywords : Bacteria; CFU; incubation.

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